Τσίκαρης Βασίλειος

Dr. VASSILIOS TSIKARIS, PROFESSOR

i.                CURRICULUM VITAE

Webpage: https://www.chem.uoi.gr/?q=el/node/61

Contact: e-mail: btsikari@uoi.gr

1958: Born in Meg. Kerassia, Kalambaka, Greece. 1977-1982: Diploma in Chemical Engineering with the distinction of “excellent” in the Institut Polytechnic “Gh. Ashaki” in Roumania. 1985-1988: Doctoral thesis entitled “Arginine Sequential Polypeptides as Histone models. Studies on their Synthesis, Conformation and Interactions with DNA. Circular Dichroism Spectroscopy”, in the Chemistry Department, Section of Organic Chemistry and Biochemistry, University of Ioannina, Greece.

Actual adress: University of Ioannina, Dept. of Chemistry, Section of Organic Chemistry and Biochemistry, 45110 Ioannina. Tel: +3026510-08383, e-mail: btsikari@uoi.gr.

Post doctoral research fellowships: 1986 (2 months), 1987 (3 months), 1988 (2 months), 1989 (6 ½ months), 1990 (2 months), 1991 (2 months), 1992 (2 ½ months), 1993 (3 months) and 1994 (5 months). Short term post doctoral research fellowships in NMR, CD, IR spectroscopy and structure-activity relationships of biomolecules,  in the «Institut National Polytechnique de Lorraine, ENSIC-INPL, CNRS UA 494, Nancy, France, supported by E.C., EMBO and FEBS.

Professional Employment:

1989-1994: Lecturer in the Chemistry Department of the University of Ioannina, Greece.

1994-1999: Assistant Professor in the same department.

1999-2006: Associate Professor in the same department

2006-Today: Professor in the same Department.

2010-2014: Head of the Department of Chemistry, University of Ioannina.

2015-2017: President of the Hellenic PASTEUR INSTITUTE.

2017-2020: Hospital Manager of the University Hospital of Ioannina

Academic Activities: Teaching Organic Chemistry, Peptide Chemistry and spectroscopy in the Chemistry Department of the University of Ioannina (1989-today) in the graduate and post-graduate courses and laboratories of Organic Chemistry (1994-today).

Supervisor of 13 concluded and 1 ongoing PhD Theses.

Supervisor of 23 concluded Masterʼs Theses.

Participation in many Advisory and Examination Committees.

Publications: In International Journals: 92. In International Books: 8. In the Proceedings of International Congresses: 78. Abstracts in International and Greek Congresses: more than 243. Citations: more than 1370.     h-index: 21.  Results from Scopus (9/2021)

Research Interests: Peptide chemistry:

1.     Design, synthesis and structure elucidation of biologically active peptides (Myasthenia gravis, Leishmania, LHRH agonists, Antithrombotic agents, cell adhesion).

2.     Development of new artificial carriers of immunogens/antigens

3.     Design and production of specific antibodies. Development of vaccines

4.     Development of new diagnostic tools.

5.     Development of new cell penetrating carriers for specific intracellular delivery of drugs.

Reviewer in a number of peer review scientific journals (Selected Journals): Journal of Combinatorial Chemistry, BBA – Proteins and Proteomics, Biopolymers, Angewandte Chemie,  Bioconjugate Chemistry, J. Peptide Sci.etc.

Oral Presentations in International and National Congresses

4  Main Lectures (Invited)

13  Short oral presentations

Participation in European and Greek funded research projects: 34

Keywords: Peptide synthesis, NMR, Circular Dichroism, Myasthenia gravis, Leishmania, GPIIb/IIIa inhibitors, Complimentary peptides, Myelopeptides, Artificial carriers, SOC carriers, immunogenic constructs.

ii.              List of publications

«Circular Dichroism Studies of Synthetic Polypeptides as Histone Models: Poly(L-Arg-L-Ile-Gly), Poly(L-Arg-L-Nva-Gly) and Poly(L-Arg-L-Nle-Gly)». V. Tsikaris, M. Sakarellos-Daitsiotis, C. Sakarellos, and M. Marraud. Biopolymers, 27, 213-229 (1988).
«Circular Dichroism Studies of Sequential Polypeptides Poly(L-Arg-X-Gly) when X=L-Ala, Val, Leu, Ile, Nva, Nle in Amphiphilic Environments». V. Tsikaris, M. Sakarellos-Daitsiotis, E. Panou-Pomonis, C. Sakarellos, and M. Marraud. Int. J. Peptide Protein Res., 33, 195-201 (1989).
«2D-NMR Study of Synthetic MIR-decapeptides Related to the Myasthenia Gravis Disease». M.T. Cung, M. Marraud, E. Bairaktari, I. Hadjidakis, V. Tsikaris, C. Sakarellos, I. Papadouli, S. Potamianos, and S.J. Tzartos. Bull. Magn. Res. 11, 381 (1989).
«Antigenic Role of Single Residues within the Main Immunogenic Region of the Nicotinic Acetylcholine Receptor». I. Papadouli, S. Potamianos, I. Hadjidakis, E. Bairaktari, V. Tsikaris, C. Sakarellos, M.T. Cung, M. Marraud, and S.J. Tzartos. Biochemical  J., 269, 239-245 (1990).
«Myasthenia Gravis, Studies with Monoclonal Antibodies against the Acetylcholine Receptor». S.J. Tzartos, M.T. Cung, A. Kordossi, H. Loutrari, M. Marraud, I. Papadouli, C. Sakarellos, D. Sophianos, and V. Tsikaris. Epitheorese klinikes farmakologias and Farmakokinetikes, International Edition, 4, 148-163, (1990).
«The main Immunogenic Region of the Acetylcholine Receptor. Structure and Role in Myasthenia Gravis». S.J. Tzartos, T. Barkas, M.T. Cung, A. Cordossi, E. Loutrari, M. Marraud, I. Papadouli, C. Sakarellos, D. Sophianos, and V. Tsikaris. Autoimmunity, 8, 259-270 (1991).
«2D-1H NMR Study of Antigen-Antibody Interactions: Binding of Synthetic Decapeptides to an Anti-Acetylcholine Receptor Monoclonal Antibody». M.T. Cung, P. Demange, M. Marraud, V. Tsikaris, C. Sakarellos, I. Papadouli, A. Kokla, and S.J. Tzartos. Biopolymers 31, 769-776 (1991).
«The Significance of Bound Residual Water in the DMSO Solution Structure of Stable Peptide Hydrates». V. Tsikaris, M. Sakarellos-Daitsiotis, N. Theophanidis, C. Sakarellos, M.T. Cung and M. Marraud. J. Chem. Soc. Perkin Trans. 2, 1353-1357 (1991).
«Changes in the CD Spectra of Calf Thymus DNA induced by Sequential Polypeptides in Aqueous Solutions. Part I». V. Tsikaris, E. Panou-Pomonis, C. Sakarellos, M. Marraud, and M. Sakarellos-Daitsiotis Int. J. Biol. Macromol. 13, 349-354 (1991).
«Changes in the CD Spectra of calf Thymus DNA Induced by Sequential polypeptides in Trifluoroethanol Solutions. Part II». E. Panou-Pomonis, V. Tsikaris, C. Sakarellos, M. Marraud and M. Sakarellos-Daitsiotis Int. J. Biol. Macromol. 13, 355-358 (1991).
«The Main Immunogenic Region (MIR) of the Nicotinic Acetylcholine Receptor and the Anti-MIR Antibodies». S.J. Tzartos, M.T. Cung, P. Demange, H. Loutrari, A. Mamalaki, M. Marraud, I. Papadouli, C. Sakarellos and V. Tsikaris. Molecular Neurobiology, 5, 1-29 (1991).
«Edude par RMN-2D des Interactions Antigene-Anticorps: Reconnaissance des Analogues Decapeptidiques du Fragment a67-76 du Recepteur RACh par les Anticorps anti-RACh». M.T. Cung, M. Marraud, V. Tsikaris, C. Sakarellos, I. Papadouli et S.J. Tzartos. J. Chim. Phys. 89, 167-173 (1992).
«2D-NMR and Molecular Dynamics Analysis of the Torpedo Californica Acetylcholine Receptor a67-76 Fragment and of its [Ala76] Analogue». M.T. Cung, V. Tsikaris, P. Demange, I. Papadouli, S.J. Tzartos, C. Sakarellos, and M. Marraud. Peptide Research, 5, 14-24 (1992).
«17O-NMR and FT-IR Study of the Ionization State of Peptides in Aprotic Solvents. Application to Leu-Enkephalin». I.P. Gerothanassis, N. Birlirakis, T. Karayannis, V. Tsikaris, M. Sakarellos-Daitsiotis, C. Sakarellos, B. Vitoux, and M. Marraud. FEBS Letters, 298, No 2,3, 188-190 (1992).
«1H-NMR Studies on Arginine Tripeptides: Evidence for Guanidinium-C-Terminal Carboxylate Interactions». V. Tsikaris, M. Sakarellos-Daitsiotis, E. Panou-Pomonis, E. Detsikas, C. Sakarellos, M.T. Cung, and M. Marraud. Peptide Res., 5, No 2, 110-114 (1992).
«The Ser-Arg-Tyr-Asp Region of the Major Surface Glycoprotein of Leishmania Mimics the Arg-Gly-Asp-Ser Cell Attachment Region of Fibronectin». K.P. Soteriadou, M.S. Remoundos, M.C. Kotsikas, A.K. Tzinia, V. Tsikaris, C. Sakarellos and S.J. Tzartos. J. Biol. Chem., 267, No20, 13980-13985 (1992).
«Interactions of an Immunogenic Decapeptide Fragment of the Neuromuscular Acetylcholine Receptor (AChR) with a Monoclonal Anti-AChR Antibody». M. Marraud, P. Demange, M.T. Cung, V. Tsikaris, C. Sakarellos, E. Vatzaki and J. Tzartos. Biochem. Soc. Trans., 20, 837-839 (1992).
«Cyclic Lactam Analogues Containing the Main Immunogenic Region (MIR) of Torpedo Acetylcholine Receptor (AChR)». E. Detsikas, V. Tsikaris, M. Sakarellos-Daitsiotis, C. Sakarellos, M.T. Cung, M. Marraud, E. Vatzaki, and S.J. Tzartos. Peptide Res., 6 No 1, 17-23 (1993).
«Conformational Requirements for Molecular Recognition of AChR MIR Analogues by Monoclonal Anti-MIR Antibody: a2D-NMR and Molecular Dynamics Approach» V. Tsikaris, E. Detsikas, M. Sakarellos-Daitsiotis, C. Sakarellos, E. Vatzaki, J. Tzartos, M. Marraud and M.T. Cung. Biopolymers, 33, 1123-1134 (1993).
«1H-NMR Studies on the Preferred Interactions of Guanidinium and C-Terminal Carboxylate Groups in Arginine Containing Peptides». V. Tsikaris, M. Sakarellos-Daitsiotis, E. Panou-Pomonis, C. Sakarellos and M.T. Cung. J. Chem. Soc. Perkin Trans. 2, 1345-1349 (1993).
«NMR Study on a SRYD-Containing Fibronectin-like Sequence (250-257) of Leishmania gp63|: Contribution of Residual Water in the Dimethyl Sulfoxide Solution Structure». V. Tsikaris, M.T. Cung, C. Sakarellos, A.K. Tzinia, K.P. Soteriadou and M. Sakarellos-Daitsiotis. J. Chem. Soc. Perkin Trans 2, 821-826 (1994).
«Conformational Properties of the Arg-Leu-Gly Tripeptide-DMSO-Water Clusters with the Combined use of Molecular Dynamics and Energy Minimization Studies». I. Demetropoulos, A. Tsibiris, V. Tsikaris, M. Sakarellos-Daitsiotis and C. Sakarellos. J. Biomol. Struct. Dynamics, 12, No 4, 755-765 (1995).
«Conformational Properties and Biological Activity of Torpedo Acetylcholine MIR-Analogues». M. Sakarellos-Daitsiotis, V. Tsikaris, E. Detsikas, C. Sakarellos, E. Vatzaki, S. Tzartos, M. Marraud and M.T. Cung. Epitheorese klinikes Farmakologias and Farmakokinetikes, International Edition, 9, 109-111, (1995).
«Antigenicity and Conformational Analysis of the Zinc-Binding Sites of two Zinc-Metalloproteases: Leishmania gp63 and Mammalian Endopeptidase-24.11». K. Soteriadou, A. Tzinia, E. Panou-Pomonis, V. Tsikaris, M. Sakarellos-Daitsiotis, C. Sakarellos, G. Papapoulou and R. Matsas. Biochemical J., 313, 455-466 (1996).
«Concept and Design of a New Class of Sequential Oligopeptide Carriers (SOC) for Covalent Attachment of Multiple Antigenic Peptides». V. Tsikaris, C. Sakarellos, M.T. Cung, M. Marraud and M. Sakarellos-Daitsiotis. Biopolymers, 38, 291-293 (1996).
«Isomerization of the Xaa-Pro peptide Bond Induced by Ionic Interactions of Arginine». V. Tsikaris, M. Sakarellos-Daitsiotis, D. Tzovaras, C. Sakarellos, P. Orlewski, M.T. Cung and M. Marraud. Biopolymers, 38, 673-682 (1996).
«Immunoreactivity and Conformation of the P-P-G-M-R-P-P Repetitive Epitope of the Sm Autoantigen». V. Tsikaris, P.G. Vlachoyiannopoulos, E. Panou-Pomonis, M. Marraud, C. Sakarellos, H.M. Moutsopoulos and M. Sakarellos-Daitsiotis. Int. J. Pept. Prot. Res., 48, 319-327, (1996).
«Construction of a New Class of Sequential Oligopeptide Carriers (SOCn) for Multiple Anchoring of Antigenic Peptides. Applications to Main Immunogenic Region (MIR) of the acetylcholine receptor (AChR)». V. Tsikaris, C. Sakarellos, M. Sakarellos-Daitsiotis, M.T. Cung, M. Marraud, E. Vatzaki and S. Tzartos. Int. J. Biol. Macromol., 19, 195-205, (1996).
«Conformational and Antigenic Properties of SRYD Containing Peptide Analogues of Leishmania gp63 Adhesion Site». M. Sakarellos-Daitsiotis, E. Panou-Pomonis, C. Sakarellos, M.T. Cung, M. Marraud, A.K. Tzinia, K. Soteriadou and V. Tsikaris. Lett. Pept. Sci., 3, 200-208 (1996).
«Compared Structures of the Free AChR MIR Decapeptide and the Antibody-Bound [A76] MIR Analogue. A Molecular Dynamics Simulation from 2D-NMR Data». P. Orlewski, M. Marraud, M.T. Cung, V. Tsikaris, M. Sakarellos-Daitsiotis, C. Sakarellos, E. Vatzaki, S.J. Tzartos. Biopolymers, 40, 419-432 (1996).
«Solution Structure of a SRYD-Containing Sequence (250-257) of the Fibronectin-like Leishmania gp63 Protein by NMR and Restrained Molecular Dynamics». P. Orlewski, V. Tsikaris, M. Sakarellos-Daitsiotis, C. Sakarellos, K.P. Soteriadou, M. Marraud and M.T. Cung. Lett. Pept. Sci., 3, 317-326, (1996).
«Use of Sequential Oligopeptide Carriers (SOCn) in the Design of Potent Leishmania gp63 Immunogenic Peptides». V. Tsikaris, C. Sakarellos, M. Sakarellos-Daitsiotis, M.T. Cung, M. Marraud, G. Konidou, A. Tzinia and K.P. Soteriadou. Peptide Research, 9, No 5, 240-247 (1996).
«Design, Synthesis and Catalytic Activity of a Serine Protease Synthetic Model».A. Stavrakoudis, I.N. Demetropoulos, C. Sakarellos, M. Sakarellos-Daitsiotis, and V. Tsikaris. Lett. Pept. Sci., 4, 481-487 (1997).
«TR-NOE and MD Studies of Leishmania GP63 SRYD-Containing Sequences Bound to Anti-SRYD Monoclonal Antibody». V. Tsikaris, M.C. Petit, P. Orlewski, M. Sakarellos-Daitsiotis, C. Sakarellos, A. Tzinia, G. Konidou, K.P. Soteriadou, M. Marraud and M.T. Cung. Lett. Pept. Sci., 4, 323-330 (1997).
«The PPGMRPP Repetitive Epitope of the Sm Autoantigen: Antigenic Specificity Induced by Conformational Changes. Application of the Sequential Oligopeptide Carriers (SOCs). C. Sakarellos, V. Tsikaris, E. Panou-Pomonis, Ch. Alexopoulos, M. Sakarellos-Daitsiotis, C. Petrovas, P.G. Vlachoyiannopoulos and M. Moutsopoulos. Lett. Pept. Sci., 4, 447-454, (1997).
«Solution Structures of the Fibronectin-Like Leishmania gp63 SRYD-containing Sequence in the Free and Antibody-Bound States. Transferred NOE and Molecular Dynamics Studies». M.-C. Petit, P. Orlewski, V. Tsikaris, M. Sakarellos-Daitsiotis, C. Sakarellos, A. Tzinia, G. Konidou, K.P. Soteriadou, M. Marraud and M.T. Cung. Eur. J. Biochem. 253, 184-193, (1998).
«A Major Sm Epitope Anchored to Sequential Oligopeptide Carriers in a Suitable Antigenic Substrate to Detect Anti-Sm Antibodies». C.J. Petrovas, P.G. Vlachoyiannopoulos, A.G. Tzioufas, Ch, Alexopoulos, V. Tsikaris, M. Sakarellos-Daitsiotis, C. Sakarellos and H.M. Moutsopoulos. J.Immunol. Methods 220, 59-68 (1998).
«Structural, Molecular and Immunological Properties of Linear B-cell Epitopes of Ro60KD Autoantigen». J.G. Routsias, M. Sakarellos-Daitsiotis, V. Tsikaris, C. Sakarellos, H.M. Moutsopoulos and A.G. Tzioufas. Scand. J. Immunol., 47, 280-287 (1998).
«The value of Synthetic Linear Epitope Analogues of La/SSB for the Detection of Autoantibodies to La/SSB. Specificity, Sensitivity and Comparison of Methods». E.E. Yiannaki, A.G. Tzioufas, M. Bachmann, J. Hantoumi, V. Tsikaris, M. Sakarellos-Daitsiotis, C. Sakarellos and H.M. Moutsopoulos. Clin. Exp. Immunol., 112, 152-158 (1998).
«Anatomy of the Antigenic Structure of a Large Membrane Autoantigen, the Muscle-Type Nicotinic Acetylcholine Receptor». S.J. Tzartos, T. Barkas, M.T. Cung, A. Mamalaki, M. Marraud, P. Orlewski, D. Papanastasiou, C. Sakarellos, M. Sakarellos-Daitsiotis, P. Tsantili and V. Tsikaris. Immunological Reviews 163, 89-120 (1998).
“Peptide Carrier: A New Helicoid-Type Sequential Oligopeptide Carrier (SOCn) for Multiple Anchoring of Antigenic Immunogenic Peptides”. M. Sakarellos-Daitsiotis, V. Tsikaris, P.G. Vlachoyiannopoulos, A.G. Tzioufas, H.M. Moutsopoulos, and C. Sakarellos. Methods 19, 133-141 (1999).
“The 3D Structure of the Complex Between an Fv Antibody Fragment and an Analogue of the Main Immunogenic Region of the Acetylcholine Receptor: A Combined 2D-NMR Homology and Molecular Modeling Approaches”. J. Kleinjung, M.-C. Petit, P. Orlewski, A. Mamalaki, S.J. Tzartos, V. Tsikaris, M. Sakarellos-Daitsiotis, C. Sakarellos, M. Marraud and M.T. Cung. Biopolymers 53, 113-128 (2000).
“A new helicoid-type sequential oligopeptide carrier (SOCn) for developing potent antigens and immunogens” M. Sakarellos-Daitsiotis, V. Tsikaris, C. Sakarellos, P.G. Vlachoyiannopoulos, A.G. Tzioufas, H.M. Moutsopoulos. Vaccine 18, 302-310 (2000).
“Arg Side-Chain-Backbone Interactions Evidenced in Model Peptides by 17O-NMR Spectroscopy”. V. Tsikaris, A. Troganis, V. Moussis, E. Panou-Pomonis, M. Sakarellos-Daitsiotis, C. Sakarellos. Biopolymers 53, 135-139 (2000).
“No Evidence of Epitope Spreading After Immunization with the Major Sm Epitope P-P-G-M-R-P-P Acnchored to Sequential oligopeptide Carriers (SOCs). P.G. Vlachoyiannopoulos, C. Petrovas, A.G. Tzioufas, Ch. Alexopoulos, V. Tsikaris, A. Guialis, L. Nakopoulou, M. Sakarellos-Daitsiotis, C. Sakarellos, P. Davaris and M. Moutsopoulos. J. Autoimmunity 14, 53-61, (2000).
“The Position of the LysNeH2-Grafted Antigens Along the Sequential Oligopeptide carrier, Ac-(Aib-Lys-Aib-Gly)n (SOCn-II), Influences the Antibody Recognition: Application to the Sm Main Autoimmune Epitope”. Ch. Alexopoulos, V. Tsikaris, C. Rizou, M. Sakarellos-Daitsiotis, C. Sakarellos, M.T. Cung, M. Marraud, P.G. Vlachoyiannopoulos, H.M. Moutsopoulos. Biopolymers 54, 1-10 (2000).
«A slow rate exchange process allows the detection of both carbonyl (C=O) and hydroxyl (-OH) 17-O nuclear magnetic resonances of the carboxylic group». V. Tsikaris, V. Moussis, M. Sakarellos-Daitsiotis and C. Sakarellos. Tetrahedron Letters 41, 8651-8654 (2000).
«A diepitopic sequential oligopeptide carrier (SOCn) as mimic of the Sm autoantigen: synthesis, conformation and biological assays». Ch. Alexopoulos, V. Tsikaris, C. Rizou, E. Panou-Pomonis, M. Sakarellos-Daitsiotis, C. Sakarellos, P.G. Vlachoyiannopoulos and H. M. Moutsopoulos. J. Peptide Sci., 7, 105-114 (2001).
«A Three Residue Cyclic Scaffold of non-RGD Containing Peptide Analogs as Platelet Aggregation Inhibitors: Design, Synthesis and Structure-Function Relationships». A. Stavrakoudis, G. Bizos, D. Eleftheriadis, A. Kouki, E. Panou-Pomonis, M. Sakarellos-Daitsiotis, C. Sakarellos, D. Tsoukatos and V. Tsikaris. Biopolymers, 56, 20-26 (2001).
«Design, Synthesis and Conformational Study of Biologically Active Photolabeled Analogues of the Main Immunogenic Region of the Acetylcholine Receptor». V. Theodorou, V. Tsikaris, M. Sakarellos-Daitsiotis, V. Avramopoulou, K. Kostelidou, S.J. Tzartos and C. Sakarellos. Biopolymers, 56, 37-46 (2001).
«A convenient and rapid method for the selective oxygen-17 enrichment of aspartyl peptides during solid-phase synthesis». V. Theodorou-Kassioumis, N. Biris, C. Sakarellos and V. Tsikaris. Tetrahedron Letters, 42, 7703-7705 (2001).
«Conformational study of the complementary peptide to a B-cell epitope of the La/SSB autoantigen». Ch. Hemmerlin, A. Phan Chan Du, Z. Elthilali, A. Moulia, V. Tsikaris, M. Sakarellos-Daitsiotis, C. Sakarellos, E. Dotsika, A.G. Tzioufas, H.M. Moutsopoulos and M.T. Cung. C. R. Acad. Sci. Paris, Chimie/Chemistry 4, 729-733 (2001).
«Unmasking the anti-La/SSB response in sera from patients with Sjogren’s Syndrome by specific blocking of anti-idiotypic antibodies to La/SSB antigenic determinants». J. G. Routsias, E. Touloupi, E. Dotsika, A. Moulia, V. Tsikaris, C. Sakarellos, M. Sakarellos-Daitsiotis, H. M. Moutsopoulos and A. G. Tzioufas. Mol. Med., 8, 293-305 (2002).
«Multiple anchoring of myelopeptides on sequential oligopeptide carriers (SOCn): synthesis, conformation and studies in human leukaemia cells». D. Keramisanou, V. Tsikaris, M. Sakarellos-Daitsiotis, C. Sakarellos, A. A. Mikhailova, L. A. Strelkov. J. Peptide Res., 60, 178-185 (2002).
«Computational screening of branched cyclic peptide motifs as potential Enzyme mimetics». A. Stavrakoudis, S. Makropoulou, V. Tsikaris , M. Sakarellos-Daitsiotis, C. Sakarellos, and I.M. Demetropoulos. J. Peptide Sci., 9, 145-155 (2003).
«The Ac-RGD-NH2 peptide as a probe of slow conformational exchange of short linear peptides in DMSO». N. Biris, A. Stavrakoudis, A.S. Politou, E. Mikros, M. Sakarellos-Daitsiotis, C. Sakarellos, and V. Tsikaris. Biopolymers, 69, 72-86, (2003).
«Mapping the binding domains of the αIIb subunit. A study performed on the activated form of the platelet integrin αIIbβ3». N. Biris, M. Abatzis, J.V. Mitsios, M. Sakarellos-Daitsiotis, C. Sakarellos, D. Tsoukatos, A.D. Tselepis, L. Michalis, D. Sideris, G. Konidou, K. Soteriadou and V. Tsikaris. Eur. J. Biochem. 270, 3760-3767, (2003).
«Aspartyl Methyl Ester Formation via Aspartimide Ring Opening: A Proposed Modification of the Protocols used in Boc- and Fmoc-Based Solid Phase Peptide Synthesis». S. Kostidis, P. Stathopoulos, N.-I. Chondrogiannis, C. Sakarellos, and V. Tsikaris. Tetrahedron Letters, 44, 8673-8676 (2003).
«Solution conformation of the antibody-bound tyrosine phosphorylation site of the nicotinic acetylcholine receptor b-subunit in its phosphorylated and nonphosphorylated states». A. Phan-Chan-Du, C. Hemmerlin, D. Krikorian, M. Sakarellos-Daitsiotis, V. Tsikaris, C. Sakarellos, M. Marinou, A. Thureau, M.T. Cung and S.J. Tzartos. Biochemistry, 42, 7371-7380 (2003).
«Synthesis and Comparison of Antibody Recognition of Conjugates Containing Herpes Simplex Virus type 1 Glycoproten D Epitope VII». G. Mezo, E. Oliveira , D. Krikorian, M. Feijlbrief, A. Jakab , V Tsikaris, C. Sakarellos, S. Welling-Wester, D. Andreu, and F. Hudecz. Bioconjugate Chemistry, 14(6), 1260-1269 (2003).
«Effect of Synthetic Peptides Corresponding to Residues 313-332 of the aIIb Subunit on Platelet Activation and Fibrinogen Binding to aIIbb3». J.V. Mitsios, A.P. Tambaki, M. Abatzis, N. Biris, M. Sakarellos-Daitsiotis, C. Sakarellos, K. Soteriadou, J. Goudevenos, M. Elisaf, D. Tsoukatos, V. Tsikaris and A.D. Tselepis. Eur. J. Biochem. 271, 855-862 (2004).
«Synthesis of a Proline-rich [60]Fullerene Peptide with Potential Biological Activity». P. Sofou, Y. Elemes, E. Panou-Pomonis, A. Stavrakoudis, V. Tsikaris, C. Sakarellos, M. Sakarellos-Daitsiotis, M. Maggini, F. Formaggio and C. Toniolo. Tetrahedron 60, 2823-2828 (2004).
«The relative orientation of the Arg and Asp side chains defined by a pseudo-dihedral angle as a key criterion for the evaluation the structure-activity relationship of RGD peptides». S. Kostidis, A. Stavrakoudis, N. Biris, D. Tsoukatos, C. Sakarellos, and V. Tsikaris. J. Peptide Sci. 10, 494-509 (2004).
«The anti-platelet approach targeting the fibrinogen ligand of the GPIIb/IIIa receptor». V. Tsikaris.  J. Peptide Sci. 10, 589–602 (2004). Review.
«a- and b- Aspartyl Peptide Ester Formation via Aspartimide Ring Opening». P. Stathopoulos, S. Papas, S. Kostidis and V. Tsikaris. J. Peptide Sci. 11, 658-664 (2005).
Invited article. «Synthetic approaches for total chemical synthesis of proteins and protein-like macromolecules of branched architecture». S. Papas, C. Strongylis and V. Tsikaris. Curr. Org. Chem.  10, 1727-744 (2006) Review.
«C-terminal N-alkylated peptide amides resulting from the linker decomposition of the Rink amide resin. A new cleavage mixture prevents their formation». P. Stathopoulos, S. Papas, S. Kostidis and V. Tsikaris. J. Peptide Sci. 12, 227-232 (2006).
«Highly constrained cyclic (S,S)-CXaaC- peptides as inhibitors of fibrinogen binding to platelets». A. Kouki, J.V. Mitsios, M. Sakarellos-Daitsiotis, C. Sakarellos, A.D. Tselepis,  V. Tsikaris and D.C. Tsoukatos. J. Thromb. Haemost. 3, 2324-2330 (2005).
«Investigation of the role of adjacent amino acids to the 313-320 sequence of the αIIb subunit on platelet activation and fibrinogen binding to aIIbb3». J.V. Mitsios, G. Stamos, L.D. Tsironis, M.-R. Stanica, C. Sakarellos, D. Tsoukatos, V. Tsikaris and A.D. Tselepis. Platelets 17(5), 277-282 (2006).
«Influence of sequential oligopeptide carriers on the bioactive structure of conjugated epitopes: Comparative study of the conformation of a Herpes simplex virus glycoprotein gD-1 epitope in the free and conjugated form, and protein “built-in” crystal structure». D. Krikorian, A. Stavrakoudis, N. Biris, C. Sakarellos, D. Andreu, E. de Oliveira, G. Mező, Z. Majer, F. Hudecz, S. Welling-Wester, M. T. Cung, and V. Tsikaris. Biopolymers 84, 383-399 (2006).
«Synthesis and antitumor activity of peptide-paclitaxel conjugates». S. Papas, T. Akoumianaki, Ch. Kalogiros, L. Hadjiarapoglou, P. A. Theodoropoulos and V. Tsikaris. J. Peptide Sci.13, 662-671 (2007).
«Assessment of the interaction between a synthetic epitope of troponin C and its specific antibody using a label-free faradaic impedimetric immunosensor and α-Keggin silicotungstic heteropolyacid as a redox probe». A. G. Mantzila, C. Strongylis, V. Tsikaris, M. I. Prodromidis. Biosensors and Bioelectronics 23(3), 362-369 (2007).
«Design, synthesis and biological evaluation of antipicornaviral pyrrole-containing peptidomimetics». I. Minchev, S. Vladimirova, L. Vezenkova, A. Bijeva, V. Moussis, L. Nikollaeva-Glomb, V. Tsikaris, M. Czeuz, and A. Galabov. Protein Pept Lett. 14(9), 917-22, (2007)
«Fasciola gigantica cathepsin L proteinase-based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis». J. Jezek, R. El Ridi, M. Salah, A. Wagih, H. W. Aziz, H. Tallima, M. H. El Shafie, T. Abdel Khalek, F. F. Abo Ammou, C. Strongylis, V. Moussis, V. Tsikaris. Biopolymers 90(3), 349-357 (2008).
«Structure-activity relationships of alpha(IIb) 313-320 derived peptide inhibitors of human platelet aggregation». M.-R. Stanica, D. Benaki, FI. Rodis, E. Mikros, D. Tsoukatos, A.D. Tselepis and V. Tsikaris. J. Pept Sci. 14, 1195-1202, (2008).
«Computational studies on the backbone-dependent side-chain orientation induced by the (S,S)-CXC motif».A. Stavrakoudis, and V. Tsikaris. J. Pept. Sci. 14, 1259–1270, (2008).
«A palmitoylated peptide, derived from the acidic carboxyl-terminal segment of the integrin IIb cytoplasmic domain, inhibits platelet activation». V. Koloka, E.D. Christofidou, S. Vaxevanelis, A.A. Dimitriou, V. Tsikaris, A.D. Tselepis, E. Panou-Pomonis, M. Sakarellos-Daitsiotis, and D.C. Tsoukatos. Platelets 19(7) 502–511, (2008).
«Effect of a Synthetic Peptide Corresponding to Residues 313 to 320 of the αIIb Subunit of the Human Platelet Integrin αIIbβ3 οn Carotid Artery Thrombosis in Rabbits». V.D. Roussa, E.M. Stathopoulou, N.D. Papamichael, C.V. Englezopoulos, P. Trypou, V. Moussis, C.C. Tellis, C.S. Katsouras, V. Tsikaris, A.D. Tselepis, L.K. Michalis. J. Pharm. Exper. Ther. 329(2), 634–640, (2009).
«Inhibition of platelet activation by peptide analogs of the beta(3)-intracellular domain of platelet integrin alpha(IIb)beta(3) conjugated to the cell-penetrating peptide Tat(48-60)». Dimitriou, A. A., Stathopoulos, P. Mitsios, J. V.,  Sakarellos-Daitsiotis, M., Goudevenos, J., Tsikaris, V., Tselepis, A. D. Platelets 20(8) 539–547, (2009).
«A highly constrained cyclic (S,S)-CDC- peptide is a potent inhibitor of carotid artery thrombosis in rabbits». Roussa, V. D.,  Stathopoulou, E. M.,  Papamichael, N. D., Englezopoulos, C. V., Rousouli, K. I.,  Trypou, P., Moussis, V., Tellis, C. C.,  Katsouras, C. S., Tsikaris, V., Tselepis, A. D., Michalis, L. K.  Platelets 22(5) 361–370, (2011).
«Artificial carriers as tool for engineering reconstituted protein mimics». Krikorian, D., Zevgiti, S. Skarlas, T., Dimtsoudi, D., Papas, S., Panou-Pomonis, E., Tsikaris, V., Sakarellos-Daitsiotis, M., Sakarellos,C. Essays on Contemporary Peptide Science, (Kordopatis,P. Ed.) 137-152, (2011).
«Side reactions in the SPPS of Cys-containing peptides». Stathopoulos P, Papas S, Pappas C, Mousis V, Sayyad N, Theodorou V, Tzakos AG, Tsikaris V. Amino Acids 44, 1357–1363(2013).
«A rapid and efficient method for the synthesis of selectively S-Trt or S-Mmt protected Cys-containing peptides». Stathopoulos  P, Papas, S, Sakka M, Tzakos GA, Tsikaris V. Amino Acids 46, 1367–1376 (2014).
«Palmitoylated peptide, being derived from the carboxyl-terminal sequence of the integrin αIIb cytoplasmic domain, inhibits talin binding to αIIbβ3». Gkourogianni A, Egot M, Koloka V, Moussis V, Tsikaris V, Panou-Pomonis E, Sakarellos-Daitsiotis M, Bachelot-Loza C, Tsoukatos DC. Platelets, 25(8), 619-627, (2014).
«Inhibition of αIIbβ3 Ligand Binding by an αIIb Peptide that Clasps the Hybrid Domain to the βI Domain of β3».  Lee WH, Schaffner-Reckinger E, Tsoukatos DC, Aylward K, Moussis V, Tsikaris V, Trypou P, Egot  M, Baruch D, Kieffer N, Bachelot-Loza C. PLoS ONE 10(9): e0134952. doi:10.1371/journal.pone.0134952, (2015).
«Molecular requirements involving the human platelet protease-activated receptor-4 mechanism of activation by peptide analogues of its tethered-ligand». Moschonas, I.C., Kellici, T.F., Mavromoustakos, T., Stathopoulos, P., Tsikaris,V., Magafa, V., Tzakos, A.G., Tselepis, A.D. Platelets, 28 (8), 812-821, (2017).
«Synergistic effect of peptide inhibitors derived from the extracellular and intracellular domain of αIIb subunit of integrin αIIbβ3 on platelet activation and aggregation». Gkourogianni, A.V., Kiouptsi, K., Koloka, V., Moussis, V., Tsikaris, V., Bachelot-Loza, C., Tsoukatos,D.C. Platelets, 29 (1), 34-4,. (2018).
«Development of novel GnRH and Tat48–60 based luminescent probes with enhanced cellular uptake and bioimaging profile». Anastasia Kougioumtzi,a Maria V. Chatziathanasiadou, Eirinaios I. Vrettos, Nisar Sayyad, Mariana Sakka, Panagiotis Stathopoulos, Michalis D. Mantzaris, Ab Majeed Ganai, Rajshekhar Karpoormath,  Georgios Vartholomatos, Vassilios Tsikaris, Theodore Lazarides, Carol Murphya and
Andreas G. Tzakos. DaltonTrans., 50, 9215 –9224, (2021).
«Multidrug resistance protein 4 (Mrp4/abcc4): A suspected efflux transporter for human’s platelet activation». Angelis I., Moussis V., Tsoukatos D.C., Tsikaris V. Protein and Peptide Letters 28(9), 983 – 995, (2021).
«Intracellular targets: A multiple cargo transporting molecule». Christos Papadopoulos, Evgenia Fotou, Vassilios Moussis, Alaxandra Ntoyhaniari, Sofia Zografou, Violetta Maltabe, Panos Kouklis, Savvas Christoforidis, Vassilios Tsikaris. J Pep Sci. 2021;27:e3359
«Control of Tyrosyl Radical Stabilization by {SiO2@Oligopeptide} Hybrid Biomimetic Materials». Panagiota Stathi, Evgenia Fotou, Vassilios Moussis, Vassilios Tsikaris, Maria Louloudi, and Yiannis Deligiannakis. Langmuir 2022 38 (32), 9799-9809 DOI: 10.1021/acs.langmuir.2c00710
«The administration of the synthetic peptide YMESRADR with a low aspirin dose significantly augments its antiplatelet effect, in contrast to the highly constrained peptide (S,S) PSRCDCR-NH2. An experimental study». Englezopoulos, K.V, Stathopoulou, E.M, Moussis, V, Chantzichristos, V., Papamichael, N.D, Tatsidou, P.T, Roussa, V., Tsoumani, M.E, Lakkas, L, Rammos, A, Katsouras, C.S, Fotou, E, Tsikaris, V., Tselepis, A.D, Michalis, Lampros. Hellenic Journal of Cardiology. (2022). 65. 10.1016/j.hjc.2022.01.002.
 

iii.            Significant research achievements in the last 10 years (publications, invited talks, patents, awards etc).

1. One of the main achievements of the VT and the Peptide Chemistry research team is the development of the innovative and multifunctional artificial carriers X-(Lys-Aib-Gly)4-NH2, and X-(Lys-Aib-Cys)4-NH2, as scaffold for the reconstitution of biomolecular (proteins, antigens, receptors) mimics. The helical conformation of the carriers (identified by 1H NMR spectroscopy and molecular modeling) mainly attributed to the inclusion of the strongly helicogenic α-aminoisobutyric acid (Aib) contributes to the optimal presentation and the initial topology of the anchored biomolecules.  These artificial carriers were extensively used in various cases as in Autoimmune diseases Sjogren Syndrom (SS), Systemic Lupus Erythematosus (SLE), Reumatoid Arthritis (RA), and Systemic Sclerosis, Myasthenia gravis, as well as for specific antibodies production and for development of diagnostic test etc.

2. Another research achievement of the VT is the development of strong inhibitors of platelet aggregation bearing the cyclic (S,S)- CDC- motif and the contribution to evaluation of the structure-activity relationships of RGD peptides. The (S,S)-CDC- sequence was incorporated, as a conformational constraint, in molecules bearing at least one positive charge with the general formula (S,S)XCDCZ where X and Z various amino acids The cyclic (S,S)- CDC- containing analogues, although resembles to RGD peptides, their  integrin binding site differs from that of the RGD peptides. Recently (2011, publication …) we have shown that these molecules are potent inhibitors of carotid artery thrombosis in rabbits. In this Topic the Patent: Cyclic peptide sequence as part of molecules that inhibit human platelet aggregation. V. Tsikaris, D. Tsoukatos, C. Sakarellos, (2002). GR 1003778 was published.

3. Over the last years our research group has made significant research efforts to identify the sequences of the platelet integrin receptor αIIbβ3 which participate in fibrinogen binding and platelet aggregation. It has been shown that the 313-332 sequence of the αIIb subunit of the human platelet integrin αIIbβ3 is involved in platelet aggregation process. Peptide analogues of this sequence have demonstrated antiaggregatory effects on human platelets in vitro. The most potent in vitro inhibitory effects on human platelet aggregation were exhibited by the peptide YMESRADR that correspond to the residues 313-320 of the αIIb subunit of the αIIbβ3 integrin. It was estimated that the peptideʼs antiaggregatory effects were exerted in a non-RGD dependent manner. Such non-RGD extracellular inhibitors possibly present more advantages than the RGD-like antagonists, as the former inhibit platelet aggregation without interacting with receptor specific sites that recognize RGD-containing sequences, and consequently they fail to induce αIIbβ3-mediated outside-in signalling. The efficacy of the peptide has been evaluated by our group on experimental model of arterial thrombosis in rabbits. This peptide significantly inhibited thrombus formation and platelet aggregation without causing haemorrhagic complications.

Patents

1.     Cyclic peptide sequence as part of molecules that inhibit human platelet aggregation.

V. Tsikaris, D. Tsoukatos, C. Sakarellos, (2002). GR 1003778.

Invited Main Lectures in International Congresses: 4

iv.   List of Research Projects

1.              “Improved method of diagnosis of allergies in antibiotics that contain β-lactamic ring”. Coordinator: Tsikaris Vassilios, Budget: 100.425,53 €, Founded by: YGEIA A.E. Duration: 01/05/2001-30/04/2003.

2.              “Development of a new immunodiagnostic assay (Elisa) for fascioliasis”. Coordinator: Tsikaris Vassilios, Budget: 13.965,00 €, Founded by:  Greek GSRT. Duration: 01/05/2003-30/04/2005.

3.              “Development of immunochemical methods for isolation, purification and determination of taxol-species from natural sources, reaction mixtures and patients serum”. Coordinator: Tsikaris Vassilios, Budget: 146.597,21 €, Founded by: Greek GSRT. Duration: 09/04/2003-08/04/2007.

4.              “Design and synthesis of RGD sequence analogues of adhesion proteins for the investigation of conformational factors that influence the biological action and specialization”. Coordinator: Tsikaris Vassilios, Budget: 33.246,58 €, Founded by: Greek Ministry of Education. Duration: 08/11/2002-31/12/2007.

5.              “Antithrombotic factors of fibrinogen. Investigation of mechanism of the inhibitory action in the aggregation of human platelets cyclic (S,S)–CDC- inhibitors and development of peptitomimetics analogues”. Coordinator: Tsikaris Vassilios, Budget: 49.942,18 €, Founded by: Greek Ministry of Education. Duration: 01/04/2005-31/12/2007.

6.              “Development of strong antiplatelet and antithrombotic peptides based on the sequences -ESRAD- and (S,S)-CDC. Investigation of suitable pharmatechnical form for in vivo use”. Coordinator: Tsikaris Vassilios, Budget: 59.500,00 €, Founded by: Greek GSRT. Duration: 01/12/2005-30/11/2007.

7.              “Effect of seasonal variations on farm fish primary and memory humoral immune responses to peptidic vaccine”. Coordinator: Tsikaris Vassilios, Budget: 8.000,00 €, Founded by: Greek GSRT. Duration: 01/04/2007-29/09/2008.

8.              “Development of reagents for detection of biochemical markers”. Coordinator: Tsikaris Vassilios, Budget: 29.500,00 €, Founded by: MEDICON HELLAS A.E. Duration: 01/01/2004-30/06/2004.

9.              “Peptide synthesis and characterization”. Coordinator: Tsikaris Vassilios, Budget: 60.000,00 €, Founded by: COMMISSION OF THE EUROPEAN COMMUNITIES, Duration: 01/12/2001-31/05/2005.

10.           “a new antiplatelet therapeutic approach for coronary syndromes: combination of peptide inhibitors of indication through antiplatelet integrin αIIbβ3 for rinical and intravenous administration”. Coordinator: Sideris Dimitrios, Budget: 1.500.000,00 €, Work package Responsible: Tsikaris Vassilios, Founded by: Greek GSRT. Duration: 01/04/2003-31/03/2006.

11.           “Interactions of molecular mechanisms of transfer signal, cytoskeleton and excretion at the thrombosis and the inflammation of endothelium”. Coordinator: Kouklis Pangiotis, Budget: 199.080,00 €, Research member: Tsikaris Vassilios, Founded by: Greek GSRT. Duration: 01/01/2006-30/06/2009.

12.           “Investigation of antithrombotic action in vivo conditions of active peptide inhibitors of platelets aggregation”. Coordinator: Michalis Lambros, Budget: 9.000,00 €, Research member: Tsikaris Vassilios, Founded by: Greek Ministry of Health. Duration: 01/10/2009-30/09/2011.

13.           “Peptides from the intracellular and extracellular region of αIIbβ3 integrin for antiplatelet action”. Coordinator: Tsoukatos Demokritos, Budget: 20.000,00 €, Research member: Tsikaris Vassilios, Founded by: Greek GSRT. Duration: 18/11/2010-17/11/2012.

14.           “Investigation of new markers for diagnosis of resistance to antiplatelet drugs in patients with cardiovascular disease. Alternative therapeutic approach to the development of new antiplatelet agents”. Coordinator: Tselepis Alexandros, Budget: 1.000.000,00 €, Work package Responsible: Tsikaris Vassilios, Budget: 80.000,00 €             , Founded by: Greek GSRT. Duration: 10/01/2012-09/01/2015.